4QQR

Structural insight into nucleotide rhamnose synthase/epimerase-reductase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	298
Detector technology	CCD
Collection date	2012-11-15
Detector	ADSC QUANTUM 1
Wavelength(s)	1.2826
Spacegroup name	P 21 21 21
Unit cell lengths	56.589, 61.943, 176.774
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.779 - 2.700
R-factor	0.2051
Rwork	0.199
R-free	0.26520
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.179
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.130
High resolution limit [Å]	2.700	7.050	2.700
Number of reflections	34737
Completeness [%]	95.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.8M ammonium sulfate, 0.1mM ZnCl2, 1mM DTT and 0.1M Hepes pH 6.5. , VAPOR DIFFUSION, SITTING DROP, temperature 277K