4QQP
Crystal structure of C1QL3 mutant D207A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.008 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 76.590, 76.590, 126.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.719 - 1.464 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.144 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.720 |
| High resolution limit [Å] | 1.460 |
| Number of reflections | 22351 |
| Completeness [%] | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292.15 | 30% PEG400, 0.1 Na-acetate, 0.01M CdCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |
