4QQ0
CdsD - The structural protein of the Type III secretion system of Chlamydia trachomatis: C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-20 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.507, 49.429, 76.927 |
| Unit cell angles | 90.00, 92.79, 90.00 |
Refinement procedure
| Resolution | 30.300 - 2.000 |
| R-factor | 0.19474 |
| Rwork | 0.192 |
| R-free | 0.24477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.973 |
| Data reduction software | PROTEUM PLUS (2) |
| Data scaling software | PROTEUM PLUS (2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.300 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 12034 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 50 mM MES; pH 6.5 - 22.5 % PEG-smear - 0.5 M ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
