4QPX

NV polymerase post-incorporation-like complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	81.220, 81.220, 188.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 1.860
R-factor	0.20725
Rwork	0.205
R-free	0.25278
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bso
RMSD bond length	0.008
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.910
High resolution limit [Å]	1.860	1.860
Rmerge	0.083	0.849
Number of reflections	73009
<I/σ(I)>	15.6	1.82
Completeness [%]	92.3	72
Redundancy	13.4	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	19 % PEG 8000, 25% glycerol, 100 mM Tris-HCl, pH 7.0, 50 mM KCl, 10 mM MnCl2 and 0.1% 2-mecaptoethanol , VAPOR DIFFUSION, HANGING DROP, temperature 293K