4QPW
BiXyn10A CBM1 with Xylohexaose Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.869, 57.049, 85.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.440 - 1.140 |
| R-factor | 0.16116 |
| Rwork | 0.160 |
| R-free | 0.17827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.361 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.069 | 1.148 |
| High resolution limit [Å] | 1.144 | 1.114 |
| Rmerge | 0.064 | 0.747 |
| Number of reflections | 65859 | |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.8 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 1.6 M Sodium citrate pH 6.5 5 mM Xylohexaose 30 mg/mL, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
