4QPR

CRYSTAL STRUCTURE OF GAF DOMAIN of POTASSIUM SENSOR HISTIDINE KINASE KDPD FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-14
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	35.310, 57.610, 61.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.500 - 1.550
R-factor	0.187
Rwork	0.185
R-free	0.20300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1230))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.500	1.590
High resolution limit [Å]	1.548	1.550
Number of reflections	18769
<I/σ(I)>	18.75
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				30-35% PEG 3350, 0.1M BIS-TRIS, pH 6.0-6.5 VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K