4QPQ

Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	110
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11587
Spacegroup name	P 43 21 2
Unit cell lengths	87.057, 87.057, 217.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.956 - 3.106
R-factor	0.2241
Rwork	0.224
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	1000.000	1000.000	3.210
High resolution limit [Å]	3.100	6.680	3.100
Rmerge	0.087	0.031	0.788
Number of reflections	15891
<I/σ(I)>	13
Completeness [%]	99.9	99.3	100
Redundancy	15.2	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1 M Tris, pH 7.0 and 17.5% PEG1000, VAPOR DIFFUSION, HANGING DROP, temperature 298K