4QPO
Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-01-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07808 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.682, 115.682, 54.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.946 - 1.999 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.082 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.073 | 0.038 | 0.740 |
| Number of reflections | 18422 | ||
| <I/σ(I)> | 14 | ||
| Completeness [%] | 99.8 | 97.9 | 99.8 |
| Redundancy | 5.5 | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1 M ammonium phosphate dibasic, 100 mM sodium citrate, pH 5.5 and 200 mM NaCl., VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
