4QP5
Catalytic domain of the antimicrobial peptidase lysostaphin from Staphylococcus simulans crystallized in the presence of phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.279, 106.780, 34.331 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 34.040 - 1.260 |
| R-factor | 0.133 |
| Rwork | 0.130 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 1.330 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Number of reflections | 64943 | |
| <I/σ(I)> | 13.23 | 2.49 |
| Completeness [%] | 98.3 | 90.9 |
| Redundancy | 5.77 | 4.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.05 M potassium dihydrogen phosphate, 20 % w/v PEG 8000 and 6 mM glycine hydroxamate supplemented with 10% (v/v) glycerol for cryocooling. Crystal was soaked for 5 min with 25 mM iodo-GGSGG pentapeptide dissolved in the buffer used for cryocooling, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
