4QOY
Novel binding motif and new flexibility revealed by structural analysis of a pyruvate dehydrogenase-dihydrolipoyl acetyltransferase sub-complex from the escherichia coli pyruvate dehydrogenase multi-enzyme complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-07-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 210.940, 326.840, 77.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.290 - 2.800 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23280 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | 2iea |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.290 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.136 | 0.605 |
| Number of reflections | 132035 | |
| <I/σ(I)> | 7.1 | 1.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.32 | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 298 | 20% PEG3350 0.2M AMMONIUM TARTRATE DIBASIC,SODIUM AZIDE, PH 6.35, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 296K, temperature 298K |
