4QOX
Crystal Structure of CDPK4 from Plasmodium Falciparum, PF3D7_0717500
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.289, 75.967, 92.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.563 - 2.748 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.450 | 2.750 |
| Rmerge | 0.143 | 0.082 | 0.902 |
| Number of reflections | 13454 | ||
| <I/σ(I)> | 5.3 | 2.86 | |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.5 | 6.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5% glycerol 1mM 3MBPP1 5 mM TCEP 28% PEG2000 MME 0.1 M BisTris pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
