4QOG
Crystal structure of fad quinone reductase 2 in complex with melatonin at 1.4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2013-03-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.560, 83.400, 106.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.810 - 1.400 |
| R-factor | 0.19295 |
| Rwork | 0.191 |
| R-free | 0.22506 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qwx |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.481 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.810 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 93437 | |
| <I/σ(I)> | 1.61 | |
| Completeness [%] | 98.0 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES PH7, 1.4M AmSO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
