4QOE
The value 'crystal structure of fad quinone reductase 2 at 1.45A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2013-03-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.410, 83.190, 106.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.970 - 1.450 |
| R-factor | 0.12814 |
| Rwork | 0.126 |
| R-free | 0.15970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qxw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 83352 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 97.4 | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES, 1.4M AmSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
