4QOA

Crystal structure of a putative periplasmic protein (BACUNI_04550) from Bacteroides uniformis ATCC 8492 at 2.75 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9116,0.9794,0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	103.311, 103.311, 90.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.823 - 2.750
R-factor	0.2166
Rwork	0.215
R-free	0.24980
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.170
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.823	29.823	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge		0.012	0.012
Total number of observations		796	5000
Number of reflections	7860
<I/σ(I)>	15.7	17.7	0.7
Completeness [%]	99.8	93.1	100
Redundancy	9.1	6.9	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	24.00% polyethylene glycol 3350, 0.16M tri-ammonium citrate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K