4QO3
Crystal structure of C16S/N18S/K12V/C117V/P134V mutant of human acidic fibroblast growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.192, 96.809, 108.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.872 - 2.047 |
| R-factor | 0.1603 |
| Rwork | 0.158 |
| R-free | 0.18720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.384 | |
| High resolution limit [Å] | 2.047 | 2.047 |
| Number of reflections | 25082 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.9 M sodium citrate, 0.1 M imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
