4QNM
CRYSTAL STRUCTURE of PSPF(1-265) E108Q MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 113.721, 113.721, 39.453 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.430 - 1.628 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bjw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.430 | 1.686 |
| High resolution limit [Å] | 1.628 | 1.628 |
| Rmerge | 0.056 | 0.891 |
| Number of reflections | 36572 | |
| <I/σ(I)> | 21.1 | 2.08 |
| Completeness [%] | 99.3 | |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 100mM Bis-Tris pH 8.0, 12-16% MPD, 2M Ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
