4QNL
Crystal structure of tail fiber protein gp63.1 from E. coli phage G7C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97969 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.773, 92.773, 551.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.959 - 2.411 |
| R-factor | 0.1624 |
| Rwork | 0.159 |
| R-free | 0.22070 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1647)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.000 | 2.560 |
| High resolution limit [Å] | 2.400 | 2.410 |
| Rmerge | 0.075 | 0.720 |
| Number of reflections | 67757 | |
| <I/σ(I)> | 15.9 | 2 |
| Completeness [%] | 99.1 | 94.4 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 150 mM Ammonium Sulphate, 34% PEE 797, 100 mM Bis-Tris pH 5.5; 1.5 ul protein @ 14.3 mg/ml + 1.5 ul well solution, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
