4QNJ
The structure of wt A. thaliana IGPD2 in complex with Mn2+ and formate at 1.3A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 114.150, 114.150, 114.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.910 - 1.300 |
| R-factor | 0.13344 |
| Rwork | 0.133 |
| R-free | 0.14355 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f1d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.510 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.064 | 0.574 |
| Number of reflections | 62158 | |
| <I/σ(I)> | 13.9 | 3 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 14% PEG200, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
