4QN5

Neuraminidase N5 binds LSTa at the second SIA binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 4
Unit cell lengths	112.251, 112.251, 66.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.960 - 1.700
R-factor	0.1201
Rwork	0.117
R-free	0.17590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sal
RMSD bond length	0.003
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	125014
Completeness [%]	95.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	291	0.1M HEPES, 12%(W/V) POLYETHYLENE GLYCOL 3350, pH 7.5, EVAPORATION, temperature 291K