4QMI

The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	64.418, 74.293, 93.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.670 - 1.900
R-factor	0.1767
Rwork	0.174
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.068
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.082	0.428
<I/σ(I)>	22.9	4.7
Completeness [%]	100.0	100
Redundancy	7.1	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	10 mg/mL protein was added to an equal volume of a mother liquor containing 28% PEG 4000, 100 mM Tris pH 7.5, and 125 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K