4QLR
Llama nanobody n02 raised against EAEC T6SS TssM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.071 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.100, 48.603, 52.872 |
| Unit cell angles | 90.00, 118.18, 90.00 |
Refinement procedure
| Resolution | 44.100 - 1.700 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4HEK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 23498 | |
| <I/σ(I)> | 9.6 | 1.7 |
| Completeness [%] | 94.3 | 90.3 |
| Redundancy | 2.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | MES 0.1M pH 6.5, PEG 8k 30% m/v, Am2SO4 0.2M , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
