4QLO

Crystal Structure of homoserine o-acetyltransferase from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 61 2 2
Unit cell lengths	49.155, 49.155, 481.417
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.24225
Rwork	0.240
R-free	0.29086
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.359
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.153	0.390
Number of reflections	12887
Completeness [%]	91.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.7 M Ammonium formate, 100 mM Imidazole hydrochloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K