4QLK
Crystal structure of rice BGlu1 E176Q/Y341A mutant complexed with cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2012-12-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.660, 79.531, 101.989 |
Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.830 |
R-factor | 0.15281 |
Rwork | 0.152 |
R-free | 0.16854 |
Structure solution method | Rigid body refinement |
Starting model (for MR) | 3F5I |
RMSD bond length | 0.008 |
RMSD bond angle | 1.280 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 87554 | |
<I/σ(I)> | 13.7 | 2 |
Completeness [%] | 98.7 | 92.8 |
Redundancy | 3.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 20% PEG MME 5000, 0.2M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K |