4QLC
Crystal structure of chromatosome at 3.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 274 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 65 |
| Unit cell lengths | 262.870, 262.870, 91.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.393 - 3.503 |
| R-factor | 0.2196 |
| Rwork | 0.218 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4INM 1hst |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.757 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.393 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.117 | 1.002 |
| Number of reflections | 45664 | |
| <I/σ(I)> | 10.2 | 1.3 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.75 | 291 | 0.1 mM Citric acid, 0.1mM potassium chloride, and 10% MPD, pH 3.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
