4QLB

Structural Basis for the Recruitment of Glycogen Synthase by Glycogenin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	94.590, 162.880, 115.790
Unit cell angles	90.00, 95.78, 90.00

Refinement procedure

Resolution	49.112 - 2.600
R-factor	0.1802
Rwork	0.179
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3naz
RMSD bond length	0.002
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1683))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.600	2.600
<I/σ(I)>	10.83	1.51
Completeness [%]	99.6	97.7
Redundancy	3.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	298	100 mM bis-Tris propane (pH 7.25), 200 mM NaSO4 and 22% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K