4QLA
Crystal structure of juvenile hormone epoxide hydrolase from the silkworm Bombyx mori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.404, 51.466, 124.449 |
| Unit cell angles | 90.00, 122.68, 90.00 |
Refinement procedure
| Resolution | 39.390 - 2.300 |
| R-factor | 0.20587 |
| Rwork | 0.203 |
| R-free | 0.25084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qo7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.108 | 0.500 |
| Number of reflections | 38204 | |
| <I/σ(I)> | 11.76 | 2.88 |
| Completeness [%] | 99.2 | 96.7 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 0.1M Tris-HCl, pH 8.0, 19% polyethylene glycol 2000, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
