4QL9
Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-182) from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 136.908, 136.908, 147.807 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.712 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 7.290 | 3.400 |
| Rmerge | 0.096 | 0.041 | 0.509 |
| Number of reflections | 22425 | ||
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 99.8 | 98.6 | 100 |
| Redundancy | 7.8 | 7.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 2M ammonium sulfate, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
