4QKM
Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under room temperature diffraction conditions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 2013-11-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.115869 |
Spacegroup name | I 4 |
Unit cell lengths | 30.090, 30.090, 67.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.001 - 1.440 |
R-factor | 0.1508 |
Rwork | 0.148 |
R-free | 0.18280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | chain A of 3C9J |
RMSD bond length | 0.019 |
RMSD bond angle | 0.948 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.480 | 1.470 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.048 | 0.531 |
Number of reflections | 5254 | |
<I/σ(I)> | 11.4 | 1.4 |
Completeness [%] | 96.7 | 98 |
Redundancy | 2.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LCP sandwich plates | 5.5 | 293 | 1.8 M calcium chloride, 0.9 M MES pH 5.5, 39.6% v/v PEG 400, 0.01M MnCl2, 4H2O additive, LCP sandwich plates, temperature 293K |