4QKM
Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under room temperature diffraction conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2013-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | I 4 |
| Unit cell lengths | 30.090, 30.090, 67.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.001 - 1.440 |
| R-factor | 0.1508 |
| Rwork | 0.148 |
| R-free | 0.18280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A of 3C9J |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.948 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.480 | 1.470 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.048 | 0.531 |
| Number of reflections | 5254 | |
| <I/σ(I)> | 11.4 | 1.4 |
| Completeness [%] | 96.7 | 98 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LCP sandwich plates | 5.5 | 293 | 1.8 M calcium chloride, 0.9 M MES pH 5.5, 39.6% v/v PEG 400, 0.01M MnCl2, 4H2O additive, LCP sandwich plates, temperature 293K |
