4QKA
c-di-AMP riboswitch from Thermoanaerobacter pseudethanolicus, iridium hexamine soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.105 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.035, 116.035, 114.098 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.009 - 3.200 |
| R-factor | 0.1739 |
| Rwork | 0.171 |
| R-free | 0.19700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 28391 | |
| Completeness [%] | 100.0 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 1.5 M (NH4)2SO4, 0.1M HEPES, pH 7.3, and 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP |
