4QK7

Influenza A M2 wild type TM domain at high pH in the lipidic cubic phase under cryo diffraction conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953724
Spacegroup name	I 4
Unit cell lengths	29.536, 29.536, 66.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.049 - 1.100
R-factor	0.1125
Rwork	0.110
R-free	0.13660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	chain A from 3C9J
RMSD bond length	0.028
RMSD bond angle	1.584
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.020	1.390
High resolution limit [Å]	0.950	1.100
Rmerge	0.083	0.246
Number of reflections	15942
<I/σ(I)>	9.9	3.16
Completeness [%]	88.2	100
Redundancy	6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Plastic LCP sandwich plates	8	283	1.8 M CaCl2, 0.9 M Tris HCl pH 8.0, 39.6% v/v PEG 400, 0.01 M beta-nicotinamide adenine dinucleotide hydrate additive, Plastic LCP sandwich plates , temperature 283K