4QJN
Crystal structure of apo nucleoid associated protein, SAV1473
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-18 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.626, 82.938, 60.717 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 30.248 - 2.613 |
| R-factor | 0.2124 |
| Rwork | 0.207 |
| R-free | 0.26340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1huu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.610 | 2.600 |
| Rmerge | 0.083 | 0.219 |
| Number of reflections | 10877 | |
| <I/σ(I)> | 33.7 | 7.2 |
| Redundancy | 6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG2000 monomethyl ether, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
