4QJD
Crystal Structure of Twister with the Nucleotide 5'- to the Cleavage Site Disordered at 3.1 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.605 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.910, 53.070, 106.750 |
| Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
| Resolution | 102.370 - 3.100 |
| R-factor | 0.19505 |
| Rwork | 0.190 |
| R-free | 0.28745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qjh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.805 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 104.828 | 50.000 | 3.220 |
| High resolution limit [Å] | 3.056 | 3.650 | 3.056 |
| Rmerge | 0.111 | 1.020 | |
| Number of reflections | 11987 | ||
| <I/σ(I)> | 7.01 | 2 | |
| Completeness [%] | 97.1 | 96.5 | 98.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 100 mM sodium acetate, pH 4.5, 200 mM ammonium sulfate, 30% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
