4QIV
Crystal structure of hexameric microcomparment shell protein from Aeromonas hydrophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .9792 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 66.460, 115.210, 122.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.170 - 2.300 |
| R-factor | 0.22381 |
| Rwork | 0.221 |
| R-free | 0.27541 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.200 | 2.360 |
| High resolution limit [Å] | 2.298 | 2.298 |
| Rmerge | 0.134 | 0.199 |
| Number of reflections | 6835 | |
| <I/σ(I)> | 8.96 | 7.85 |
| Completeness [%] | 64.0 | 14.8 |
| Redundancy | 6.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPES, 1.5M Ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
