4QIO
Lambda-[Ru(TAP)2(dppz)]2+ bound to d(TCGGCGCCIA) at high resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.82565 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.160, 42.160, 39.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.828 - 0.950 |
| R-factor | 0.09655 |
| Rwork | 0.095 |
| R-free | 0.11928 |
| Structure solution method | PDB ENTRY 3QRN |
| RMSD bond length | 0.021 |
| RMSD bond angle | 3.084 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.828 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Number of reflections | 22994 | |
| Completeness [%] | 99.5 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1 uL 1 mM d(TCGGCGCCIA)2 + 1 uL 4 mM rac-Ru(TAP)2(dppz).2Cl + 6uL 40 mM sodium cacodylate, 12 mM spermine, 10% MPD, 20 mM barium chloride, 80 mM potassium chloride equilibrated against 1 mL 35% MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
