4QIG
Crystal Structure of PduA with edge mutation K26A and pore mutation S40C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 235.440, 235.440, 235.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.240 - 3.297 |
| R-factor | 0.1932 |
| Rwork | 0.189 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.889 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.240 | 3.380 |
| High resolution limit [Å] | 3.297 | 3.297 |
| Rmerge | 0.163 | |
| Number of reflections | 16403 | |
| <I/σ(I)> | 32.47 | 4.24 |
| Completeness [%] | 99.7 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.5M Ammonium sulfate, 0.1M HEPES pH 7.5, 30% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
