4QI4
Dehydrogenase domain of Myriococcum thermophilum cellobiose dehydrogenase, MtDH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 63 |
| Unit cell lengths | 171.755, 171.755, 72.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.320 - 2.700 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1477)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.320 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 33521 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 298 | 0.1 M NaAc pH 4.6, 0.1 M CdCl2, 18% (w/v) PEG monomethylether 550, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
