4QI3
Cytochrome domain of Myriococcum thermophilum cellobiose dehydrogenase, MtCYT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.405, 56.357, 73.005 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 44.060 - 1.400 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BALBES |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.483 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.820 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 75847 |
| Completeness [%] | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 298 | 0.1 M Tris-HCl pH 8.4, 0.2 M MgCl2, 30% (w/v) PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
