4QI2
X-ray structure of the ROQ domain from murine Roquin-1 in complex with a 23-mer Tnf-CDE RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.560, 60.410, 84.370 |
| Unit cell angles | 105.68, 101.36, 95.72 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.20549 |
| Rwork | 0.203 |
| R-free | 0.24679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qi0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.513 |
| Number of reflections | 19951 | |
| <I/σ(I)> | 13.9 | 3 |
| Completeness [%] | 95.7 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 200 mM NaCl, 100 mM Bis-Tris, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
