4QI0
X-ray structure of the ROQ domain from murine Roquin-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.90745 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.220, 79.510, 184.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.460 - 1.940 |
| R-factor | 0.16327 |
| Rwork | 0.161 |
| R-free | 0.20556 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.880 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.137 | 0.621 |
| Number of reflections | 30555 | |
| <I/σ(I)> | 9.7 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 300 mM Na Tartrate, 100 mM Bis-Tris-Propane, 20% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
