4QHS

Crystal structure of AAA+sigma 54 activator domain of the flagellar regulatory protein FlrC of Vibrio cholerae in nucleotide free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	81.292, 153.285, 193.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.387 - 2.300
R-factor	0.1932
Rwork	0.192
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dzd
RMSD bond length	0.008
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.387	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	96306
Completeness [%]	89.0	89.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% PEG 6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K