4QHH
Crystal structure of Methanocaldococcus jannaschii tetrameric selecase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 102.220, 102.220, 59.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.300 - 3.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dimeric selecase |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 49.300 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.058 | 0.058 |
| Number of reflections | 3962 | |
| <I/σ(I)> | 32.4 | 32.4 |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Bis-Tris propane, 0.2M sodium citrate, 18-24%(w/v) polyethylene glycol 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
