4QGY
Camelid (llama) nanobody n25 (VHH) against type 6 secretion system TssM protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 51.980, 70.950, 145.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.150 - 1.380 |
| R-factor | 0.1915 |
| Rwork | 0.191 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hep |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.031 | 0.700 |
| Number of reflections | 55440 | |
| <I/σ(I)> | 28.7 | 2.63 |
| Completeness [%] | 99.6 | 95 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | KNa Tartrate 1.2M, CHES 0.1M, Li2SO4 0.2M, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
