4QGU

protein domain complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	85.715, 91.865, 69.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.399 - 2.545
R-factor	0.2387
Rwork	0.238
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.528
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.399
High resolution limit [Å]	2.545
Number of reflections	18078
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% (w/v) PEG 3350 and 0.2 M potassium fluoride; 20% (w/v) PEG 3350 and 0.2 M sodium formate; or 20% (w/v) PEG 3350 and 0.2 M lithium acetate dehydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K