4QG5
Crystal structure of phosphoglucomutase from Leishmania major at 3.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-18 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.358, 114.971, 125.915 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 38.167 - 3.500 |
| R-factor | 0.2417 |
| Rwork | 0.238 |
| R-free | 0.31470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pmg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.655 | 3.710 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.148 | 0.512 |
| Number of reflections | 29659 | |
| <I/σ(I)> | 8.3 | 2.3 |
| Completeness [%] | 86.6 | 88.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 0.1M Tris-HCl, pH 8.0, 10% (w/v) PEG 3350, 10mM MgCl2, 0.02% Sodium Azide, Hanging drop vapor diffusion method, temperature 293.0K |
