4QFV
Crystal structure of a unique ankyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.669, 92.748, 69.636 |
| Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
| Resolution | 48.735 - 1.999 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o60 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 56375 | |
| <I/σ(I)> | 18.1 | 2.63 |
| Completeness [%] | 98.5 | 96.2 |
| Redundancy | 7.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100 MM SODIUM ACETATE TRIHYDRATE (PH 4.5), 200 MM (NH4)2SO4, 16-18% PEG 3350, AND 10% GLYCEROL, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
