4QFK

Crystal structure of dipeptide binding protein from pseudoalteromonas sp. SM9913

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	93.386, 93.970, 140.549
Unit cell angles	80.03, 84.43, 69.84

Refinement procedure

Resolution	42.629 - 2.288
R-factor	0.1946
Rwork	0.191
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpe
RMSD bond length	0.008
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MLPHARE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.288	2.300
Number of reflections	185390
Completeness [%]	95.1	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9.2	277	0.2M potassium phosphate dibasic, 20%(w/v) polyethylene glycol 3350, pH 9.2, EVAPORATION, temperature 277K