4QEG

Crystal structure of domain I10 from titin (space group P41)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9464
Spacegroup name	P 41
Unit cell lengths	31.237, 31.237, 93.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.760 - 2.000
R-factor	0.1877
Rwork	0.183
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	5665
<I/σ(I)>	4.96	2.34
Completeness [%]	93.2	91.9
Redundancy	2.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		0.2 M CaCl2, 30% PEG 3350, 0.1 M Tris, 3% Isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP