4QEC
ElxO with NADP Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97624 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.503, 57.234, 82.317 |
| Unit cell angles | 90.00, 121.04, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| R-factor | 0.16314 |
| Rwork | 0.161 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 40552 | |
| <I/σ(I)> | 23.4 | 6 |
| Completeness [%] | 97.8 | 86 |
| Redundancy | 6.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 0.1 M ammonium acetate, 0.02 M magnesium chloride 0.05 M HEPES-Na, 8% (v/v) PEG 800, 5% (v/v) glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
