4QE9
Open MthK pore structure soaked in 10 mM Ba2+/100 mM K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 61.005, 61.005, 44.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.500 - 2.150 |
| R-factor | 0.20392 |
| Rwork | 0.198 |
| R-free | 0.25673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.105 |
| Number of reflections | 4888 |
| <I/σ(I)> | 26.6 |
| Completeness [%] | 98.4 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 3.0-3.5M 1,6-Hexandiol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
