4QDN
Crystal Structure of the endo-beta-N-acetylglucosaminidase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.560, 61.194, 101.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.054 - 1.700 |
| R-factor | 0.1675 |
| Rwork | 0.167 |
| R-free | 0.19360 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | MOSFLM |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.600 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.585 | |
| Number of reflections | 16057 | |
| <I/σ(I)> | 13.5 | 2.3 |
| Completeness [%] | 98.6 | 91 |
| Redundancy | 7.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 50% MPD, 0.1 M Tris-HCl, 0.2 M ammonium phosphate monobasic, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
